The plate reader optics are tunable for. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. It measures the optical density (OD) of samples in both 96- and 384-well. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. 012 fmol 0. 0 out of 5 $ 5,999. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. See SpectraMax® Microplate Reader User Manual Download Page. 0 out of 5 $ 23,995 $ 22,495. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. We would like to show you a description here but the site won’t allow us. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. 0 out of 5 $ 9,995 $ 9,495. 7°C (gradient heating design). The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 5 87. ATP standard curves read on SpectraMax microplate readers. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 305 reference level saturation. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. SpectraMax Microplate Readers. Selection of Water Constant in PathCheck settings. Selection of Water Constant in PathCheck settings. Access this by going to Help >. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. 314 low lamp level warning. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Open the top cover of the instrument. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax® iD3 Multi Mode Microplate . SpectraMax Mini Plug-In for SoftMax Pro 7. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. In addition, the SpectraMax® iD5 can be expanded to include bottom. . Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Fully tested with validations, warranty. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Summary of Contents for Molecular Devices SpectraMax M2. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Table 2 summarizes the results for the uniformity test. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. flexibility of monochromator-based systems. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Five preferred detection modes. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. Download PDF. zip. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. 0 out of 5 $ 5,999. A preconfigured protocol is available in the software to. In addition, the SpectraMax iD5 can be expanded to. Microplate reader settings. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Sale. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. SpectraMax Spectrofluorometers. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). Time-. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SpectraMax® ABS Plus Microplate Reader can run . The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Ease of use. Detailed specifications of each model reader are reported in Section 7, “Specifications”. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Highly customizable low volume. Find regional support. The data suggests that there is little interference from dead cells when live cells are analyzed, however, there may be some interference from live cells when dead cells are analyzed. Intended Use/Discipline:Biological. Published Dec 16, 2020 | Updated May 19, 2023. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Cell Cytotoxicity assay procedure. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 997 (n = 3). Category 2 and 3 microplate readers use gradient heating as well. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. Absorbance (optical density, OD) was read at 570 nm and 605. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. both cuvette-based and microplate reader applications on the same instrument. Download the SpectraMax Mini Reader Plug-in and associated guides. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. SpectraMax Spectrofluorometers. Example: [Drawer]. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. SpectraMax Plus: SpectraMax Plus. Temperature control and shaking come as standard. limit of detection (LLD) for ATP was estimated for each microplate reader using the . The system uses two scanning monochromators to determine the optimal excitation and emission settings. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . . Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. Introduction. Compact and tunable absorbance microplate readers DATA SHEET. Add to Cart. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. 100 fmol 384-well (75 µL) 0. Next generation SpectraMax® absorbance microplate readers. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). Find regional support. Dual-Glo luciferase assay system (Promega Cat. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The cuvette must be in place when you read the plate. This pathlength correction is accomplished only with the use of. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. 3) This warranty. It features a modular design that allows users to add or change detection cartridges according to their needs. Microplate automation and detection instruments for walk away operation and high quality results. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Path Check Pathlength Measurement SpectraMax i3x. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. On the back, in the right hand top corner,. 58% for the detection module and 2. both cuvette-based and microplate reader applications on the same instrument. 0 out of 5 $ 11,999. For a higher . SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 1. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. limit of detection (LLD) for ATP was estimated for each microplate reader using the . The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 0200-6263. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Cuvette Microplate wells. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. It can run samples based on pre-defined. Most papers they cultured bacteria to OD600>0. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Table of Contents. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. . The instrument uses a paired monochromator and xenon flash. 4 μL DNA standards. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). The University of Rouen in Normandy uses the SpectraMax iD3 and. The guide also covers the software features, the specifications, and the accessories of the systems. In . With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. no. Measuring platelet aggregation with a microplate reader. Contact our support team with your questions regarding Molecular Devices products and applications. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. Read More. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Turn the reader off by pulling the power cord. 470 nm). Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. 76358-624. Detailed specifications of each model reader are reported in Section. Step 1: Turn on your microplate reader. Italic and boldface type are used for emphasis. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. We would like to show you a description here but the site won’t allow us. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. 030 fmol Fluorescence Polarization (FP). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Table 1. no. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. Add to Cart. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. No additional manuals are available. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Molecular Devices. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Type: Clear: Add to Cart. Products : General, Microplate Readers. Step 1: Open the SoftMax Pro IMAP FP protocol. Table of Contents. The drawing shows a standard microplate of 15 mm height covered with a lid. Example: [Drawer]. their consistent performance, reliability and durability. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. The price range of a reader starts at 2. ATP standard curves read on SpectraMax microplate readers. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. 5 12. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. 7°C and the bottom to 36. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. 003 fmol 0. 380 nm/Exc. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. 313 reference gain check fail. All stand-alone instrument functions can be controlled using the software. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. In addition, the SpectraMax® iD5 can be expanded to include bottom. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Step 1: Turn on your microplate reader. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Transcreener ® ADP. The SpectraMax M2 and M2e are versatile and reliable. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). If it looks fine, then proceed. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. both cuvette-based and microplate reader applications Microplate Readers. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. These lab workhorses are used in drug discovery. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The plate. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax M5 reader settings are shown. 1) Select the correct plate and the wells that you want to measure. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . SpectraMax 250 User. 0 out of 5 $ 5,999. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Microplate Spectrophotometers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. We would like to show you a description here but the site won’t allow us. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Measure more accurately. The guide also covers the software features, the specifications, and the accessories of the systems. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Download PDF. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 304 signal level saturation. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. For SpectraMax ABS Plus reader, r 2 = 0. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. We use Spectramax M5 microplate reader in our lab. Figure 6: Transcreener ADP2 FI assay principle. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. It also integrates with the. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. The plate reader can measure multiple readouts, including UV and visible absorbance. To resume collecting kinetic measurements within the same file, press the Append button. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. 2. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. . The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. In addition, the iD5 reader measures TRF and FP. 304 signal level saturation. Figure 3. Supplier: Molecular Devices. These robust readers have been placed in labs from. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. . SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. Use the provided 3. Wide range of applications The VersaMax reader covers. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. 3) At the bottom of the strip, click on "Show. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. 0 out of 5 $ 6,499. Absorbance (optical density, OD) was read at 570 nm and 605. Positions H21-K22 contained plate blanks of PBS only. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. 2. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. Nucleic Acid and Protein Quantitation. Here, the CVs for each plate were less than 3, and the difference between mean. 0 out of 5 $ 2,999-20%. SAN JOSE, CA — Molecular Devices, LLC. Molecular Devices SpectraMax M2E Microplate Reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Microplate Readers. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. SAN JOSE, CA — Molecular Devices, LLC. SoftMax® Pro 7. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. 1. The 2-channel configuration is available with 0, 2, or 4 injectors. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 1. The plate reader optics are tunable for absorbance. Endpoint, kinetic, spectrum. A: Microplate Preparation. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader.